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TOLLIP Polyclonal antibody

TOLLIP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11315-1-AP

Synonyms

IL 1RAcPIP, toll interacting protein, TOLLIP

Validation Data Gallery

  • IHC staining of human gliomas using 11315-1-AP

    IHC staining of human gliomas using 11315-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11315-1-AP (TOLLIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 11315-1-AP

    IHC staining of human gliomas using 11315-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11315-1-AP (TOLLIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of PC-3 using 11315-1-AP

    WB analysis of PC-3 using 11315-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 11315-1-AP

    IF Staining of PC-3 using 11315-1-AP

    Immunofluorescent analysis of PC-3 cells using 11315-1-AP (TOLLIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, human liver tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11315-1-AP targets TOLLIP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOLLIP fusion protein Ag1864
Full Name toll interacting protein
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC012057
Gene symbol TOLLIP
Gene ID (NCBI) 54472
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOLLIP is a component of the signaling pathway of IL-1 and Toll-like receptors. It controls the magnitude of inflammatory cytokine production in response to IL-1B and LPS.

Protocols

Product Specific Protocols
WB protocol for TOLLIP antibody 11315-1-APDownload protocol
IHC protocol for TOLLIP antibody 11315-1-APDownload protocol
IF protocol for TOLLIP antibody 11315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols