TOLLIP Polyclonal antibody
TOLLIP Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11315-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, HEK-293 cells, human liver tissue, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | PC-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
11315-1-AP targets TOLLIP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TOLLIP fusion protein Ag1864 |
Full Name | toll interacting protein |
Calculated molecular weight | 30 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC012057 |
Gene symbol | TOLLIP |
Gene ID (NCBI) | 54472 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TOLLIP is a component of the signaling pathway of IL-1 and Toll-like receptors. It controls the magnitude of inflammatory cytokine production in response to IL-1B and LPS.
Protocols
Product Specific Protocols | |
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WB protocol for TOLLIP antibody 11315-1-AP | Download protocol |
IHC protocol for TOLLIP antibody 11315-1-AP | Download protocol |
IF protocol for TOLLIP antibody 11315-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |