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TPST1 Polyclonal antibody

TPST1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12404-1-AP

Synonyms

TPST 1, TPST1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TPST1 Polyclonal antibody (12404-1-AP)

    WB analysis using 12404-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12404-1-AP (TPST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPST1 Polyclonal antibody (12404-1-AP)

    IHC staining of human colon cancer using 12404-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12404-1-AP (TPST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPST1 Polyclonal antibody (12404-1-AP)

    IHC staining of human colon cancer using 12404-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12404-1-AP (TPST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12404-1-AP targets TPST1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPST1 fusion protein Ag3093
Full Name tyrosylprotein sulfotransferase 1
Calculated Molecular Weight 370 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC013188
Gene Symbol TPST1
Gene ID (NCBI) 8460
RRIDAB_2877855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TPST1 antibody 12404-1-APDownload protocol
IHC protocol for TPST1 antibody 12404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
hamsterWB

Biotechnol J

Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation.

Authors - Ren Liu
View Article