Tested Applications
| Positive WB detected in | A549 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12404-1-AP targets TPST1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3093 Product name: Recombinant human TPST1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-371 aa of BC013188 Sequence: ECHHRIEERSQPVKLESTRTTVRTGLDLKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLSRLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKIIENTRRVYKGEFQLPDFLKEKPQTEQVE Predict reactive species |
| Full Name | tyrosylprotein sulfotransferase 1 |
| Calculated Molecular Weight | 370 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC013188 |
| Gene Symbol | TPST1 |
| Gene ID (NCBI) | 8460 |
| RRID | AB_2877855 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60507 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TPST1 is a human tyrosylprotein sulfotransferase that uses 3′phosphoadenosine-5′phosphosulfate (PAPS) to transfer the sulfate moiety to proteins predominantly designated for secretion. High TPST1 expression was linked with clinicopathological characteristics and unfavorable prognosis of HNSC patients (PMID: 16859706). In lung cancer, TPST1 expression was associated with the tumor-node metastasis (TNM) stage and lymph node metastasis, suggesting that it may be a useful prognostic indicator for lung cancer patients (PMID: 18243191, 17028309).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TPST1 antibody 12404-1-AP | Download protocol |
| WB protocol for TPST1 antibody 12404-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TPST1 Polyclonal antibody (Cat# 12404-1-AP) has 1 total citation. It appears in Biotechnology Journal (2021), in a study on modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation. The associated research field is biotechnology and protein engineering, specifically antibody production and post-translational modification.
| Species | Application | Title |
|---|---|---|
Biotechnol J Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation. |
