TRIM47 Polyclonal antibody

TRIM47 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26885-1-AP

Synonyms

GOA, RING finger protein 100, RNF100, TRIM47, tripartite motif containing 47



Tested Applications

Positive WB detected inU2OS cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman kidney tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26885-1-AP is TRIM47 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM47 fusion protein Ag22868
Full Name tripartite motif-containing 47
Calculated molecular weight 638 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC017299
Gene symbol TRIM47
Gene ID (NCBI) 91107
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for TRIM47 antibody 26885-1-APDownload protocol
IHC protocol for TRIM47 antibody 26885-1-APDownload protocol
IP protocol for TRIM47 antibody 26885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols