- Featured Product
- KD/KO Validated
TRIM47 Polyclonal antibody
TRIM47 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 26885-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | U2OS cells |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human kidney tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 6 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 26885-1-AP is TRIM47 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIM47 fusion protein Ag22868 |
| Full Name | tripartite motif-containing 47 |
| Calculated Molecular Weight | 638 aa, 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC017299 |
| Gene Symbol | TRIM47 |
| Gene ID (NCBI) | 91107 |
| RRID | AB_2880669 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM47 antibody 26885-1-AP | Download protocol |
| IHC protocol for TRIM47 antibody 26885-1-AP | Download protocol |
| IP protocol for TRIM47 antibody 26885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.
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In Vitro Cell Dev Biol Anim TRIM47 promotes glioma angiogenesis by suppressing Smad4
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Clinics (Sao Paulo) TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling
| ||
Exp Brain Res Knocking down Trim47 ameliorated sevoflurane-induced neuronal cell injury and cognitive impairment in rats
| ||
Comput Struct Biotechnol J Featured interactome of homocysteine-inducible endoplasmic reticulum protein uncovers novel binding partners in response to ER stress |
