• Featured Product
  • KD/KO Validated

TRMT11 Polyclonal antibody

TRMT11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17555-1-AP

Synonyms

tRNA guanosine-2'-O-methyltransferase TRM11 homolog, tRNA (guanine(10)-N2)-methyltransferase homolog, TRM11, MDS024, EC:2.1.1.-



Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, LNCaP cells, K-562 cells, mouse testis tissue, HepG2 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17555-1-AP targets TRMT11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRMT11 fusion protein Ag11717
Full Name tRNA methyltransferase 11 homolog (S. cerevisiae)
Calculated Molecular Weight 463 aa, 53 kDa
Observed Molecular Weight 48-53 kDa
GenBank Accession NumberBC056878
Gene Symbol TRMT11
Gene ID (NCBI) 60487
RRIDAB_2209511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRMT11 antibody 17555-1-APDownload protocol
IHC protocol for TRMT11 antibody 17555-1-APDownload protocol
IF protocol for TRMT11 antibody 17555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Microbiol Immunol

BUD23-TRMT112 interacts with the L protein of Borna disease virus and mediates the chromosomal tethering of viral ribonucleoproteins.

Authors - Bea Clarise B Garcia