Tested Applications
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83410-4-RR targets TRNP1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag22504 Product name: Recombinant human TRNP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC069216 Sequence: MPGCRISACGPGAQEGTAEQRSPPPPRDPMPSSQPPPPTPTLTPTPTPGQSPPLPDAAGASAGAAEDQELQRWRQGASGIAGLAGPGGGSGAAAGAGGRALELAEARRRLLEVEGRRRLVSELESRVLQLHRVFLAAELRLAHRAESLSRLSGGVAQAELYLAAHGSRLKKGPRRGRRGRPPALLASALGLGGCVPWGAGRLRRGHGPEPDSPFRRSPPRGPASPQR Predict reactive species |
| Full Name | TMF1-regulated nuclear protein 1 |
| Calculated Molecular Weight | 227 aa, 23 kDa |
| GenBank Accession Number | BC069216 |
| Gene Symbol | TRNP1 |
| Gene ID (NCBI) | 388610 |
| RRID | AB_3671058 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q6NT89 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TRNP1 antibody 83410-4-RR | Download protocol |
| IF protocol for TRNP1 antibody 83410-4-RR | Download protocol |
| IHC protocol for TRNP1 antibody 83410-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
