Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | pig brain tissue |
| Positive IHC detected in | human gliomas, mouse testis tissue, human ovary cancer tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26973-1-AP targets TTYH1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25290 Product name: Recombinant human TTYH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 264-390 aa of BC011347 Sequence: EAATAVGLSDFCSNPDPYVLNLTQEETGLSSDILSYYLLCNRAVSNPFQQRLTLSQRALANIHSQLLGLEREAVPQFPSAQKPLLSLEETLNVTEGNFHQLVALLHCRSLHKDYGAALRGLCEDALE Predict reactive species |
| Full Name | tweety homolog 1 (Drosophila) |
| Calculated Molecular Weight | 49-50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC011347 |
| Gene Symbol | TTYH1 |
| Gene ID (NCBI) | 57348 |
| RRID | AB_2880707 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H313 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TTYH1, protein tweety homolog 1, is a glycosylated protein composed of five predicted transmembrane segments, which belongs to the tweety family. The expression of TTYH1 is restricted mainly to neural tissue and is up-regulated in astrocytoma, glioma and several other cancers. TTYH1 has some isoforms with MW of 49-50 kDa, 35 kDa and 26 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TTYH1 antibody 26973-1-AP | Download protocol |
| IP protocol for TTYH1 antibody 26973-1-AP | Download protocol |
| WB protocol for TTYH1 antibody 26973-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TTYH1 Polyclonal Antibody (Cat# 26973-1-AP) has a total of 2 citations. These appear in Nature Communications and Journal of Cancer. The cited research spans two fields: one study investigates endolysosomal lipid processing and autophagy in astrocytes (neuroscience), while the other examines TTYH1 expression as a prognostic marker in ovarian cancer (oncology). The antibody was used for IF in the neuroscience study and for WB and IHC in the cancer study, with reactivity confirmed in both mouse and human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Endolysosomal processing of neuron-derived signaling lipids regulates autophagy and lipid droplet degradation in astrocytes | ||
J Cancer Elevated expression of Tweety homologue 3 predicts poor clinical outcomes in ovarian cancer. |
