TUBB3-specific Monoclonal antibody

TUBB3-specific Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F8G10

Cat no : 66375-1-Ig

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3, Tuj1



Tested Applications

Positive WB detected infetal human brain tissue, SH-SY5Y cells
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, iPS cells, rat brain tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66375-1-Ig targets TUBB3-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, beta 3
Calculated molecular weight 55 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberNM_001197181
Gene symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.

Protocols

Product Specific Protocols
WB protocol for TUBB3-specific antibody 66375-1-IgDownload protocol
IHC protocol for TUBB3-specific antibody 66375-1-IgDownload protocol
IF protocol for TUBB3-specific antibody 66375-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biochem Biophys Res Commun

Functional analysis of juxta- and intra-membrane domains of murine APP by genome editing in Neuro2a cells.

Authors - Nanaka Kaneshiro
humanIF

Stem Cells Dev

Zinc Promotes Patient-Derived Induced Pluripotent Stem Cell Neural Differentiation via ERK-STAT Signaling.

Authors - Meng Yang
humanIF

CNS Neurosci Ther

A single factor induces neuronal differentiation to suppress glioma cell growth.

Authors - Ji-Qiang Fu
mouseIF

Drug Deliv

PMID: 32133894

Authors - Tingting Li
humanWB

Cell Physiol Biochem

A Human Long Non-Coding RNA ALT1 Controls the Cell Cycle of Vascular Endothelial Cells Via ACE2 and Cyclin D1 Pathway.

Authors - Wen Li
mouseFC

Front Cell Neurosci

PMID: 28729825

Authors - Cong Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Josh (Verified Customer) (12-18-2018)

BIII tubulin antibody signal nicely fills neuronal cell bodies and processes with good signal to noise at a reasonable dilution (1:500 in 1% NDS, 0.3% Triton-X, PBS with donkey anti-mouse 647 secondary, 1:500). However, BIII tubulin antibody signal also colocalized with S100 immunoreactive astrocytic processes, though with a lesser intensity. Overall, the antibody is a useful marker of neuronal differentiation when used in combination with other cell-selective antibodies and morphology. Image caption: Differentiated adult hippocampal neural stem cell culture. BIII tubulin 1:500 (purple), S100 1:500 (red), Hoechst (blue). Scale bar = 20 um.

  • Applications: Immunofluorescence,Cell culture,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Adult hippocampal neural stem cells, differentiated for 7 days
TUBB3-specific Antibody Immunofluorescence,Cell culture, validation (1:500 dilution) in Adult hippocampal neural stem cells, differentiated for 7 days (Cat no:66375-1-Ig)