TUBGCP5 Polyclonal antibody

TUBGCP5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14620-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Gamma-tubulin complex component 5, GCP5, GCP-5, KIAA1899

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 14620-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14620-1-AP (TUBGCP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 14620-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14620-1-AP (TUBGCP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 14620-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14620-1-AP (TUBGCP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 14620-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14620-1-AP (TUBGCP5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 14620-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14620-1-AP (TUBGCP5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 14620-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14620-1-AP (TUBGCP5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected in	human rectal cancer tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14620-1-AP targets TUBGCP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6248 Product name: Recombinant human TUBGCP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 676-1024 aa of BC071560 Sequence: ESVTCQTFELTLRSCLYPHIDKQYLDCCGNLMQTLKKDYRLVEYLQAMRNFFLMEGGDTMYDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSSRLSISFENVDTAKKKLPVHILDGLTLSYKVPWPVDIVISLECQKIYNQVFLLLLQIKWAKYSLDVLLFGELVSTAEKPRLKEGLIHEQDTVAQFGPQKEPVRQQIHRMFLLRVKLMHFVNSLHNYIMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRCLLREKVSFVKEAIMKVLNLALMFADGWQAGLGTWRMESIEKMESDFKNCHMFLVTILNKAVCRGSFPHLESLALSLMAGMEQS Predict reactive species
Full Name	tubulin, gamma complex associated protein 5
Calculated Molecular Weight	118 kDa
Observed Molecular Weight	118-120 kDa
GenBank Accession Number	BC071560
Gene Symbol	TUBGCP5
Gene ID (NCBI)	114791
RRID	AB_2878070
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96RT8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TUBGCP5 antibody 14620-1-AP	Download protocol
WB protocol for TUBGCP5 antibody 14620-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TUBGCP5 Polyclonal antibody (Cat# 14620-1-AP) has a total of 1 citation. It appears in Cell Death & Differentiation (IF: 13.6), published in 2019. The cited study investigated how haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in the retina, placing this citation in the research fields of autophagy, cytoskeletal dynamics, and retinal degenerative disease.

Species	Application	Title
mouse	WB	Cell Death Differ Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina. Authors - Zhigang Li View Article