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  • KD/KO Validated

TWIST1-specific Polyclonal antibody

TWIST1-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 25465-1-AP

Synonyms

ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1



Tested Applications

Positive WB detected inMDA-MB-453s cells, A549 cells, mouse brain tissue, Raji cells
Positive IP detected inMDA-MB-453s cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25465-1-AP is TWIST1-specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TWIST1-specific fusion protein Ag21924
Full Name twist homolog 1 (Drosophila)
Calculated molecular weight 202 aa, 21 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC036704
Gene symbol TWIST1
Gene ID (NCBI) 7291
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with twist1 protein.

Protocols

Product Specific Protocols
WB protocol for TWIST1-specific antibody 25465-1-APDownload protocol
IHC protocol for TWIST1-specific antibody 25465-1-APDownload protocol
IF protocol for TWIST1-specific antibody 25465-1-APDownload protocol
IP protocol for TWIST1-specific antibody 25465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Metab

ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition.

Authors - Ming Lu
  • KD Validated
humanWB

Cancer Cell Int

SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition.

Authors - Zhou Yang
humanWB

Biochem Cell Biol

The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway.

Authors - Sijia Han
humanWB

J Cell Physiol

Comparison of phenotypic markers and neural differentiation potential of human bone marrow stromal cells from the cranial bone and iliac crest.

Authors - Yuyuan Ma
human

Theranostics

Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme.

Authors - Yaodong Chen
humanWB

Oncol Lett

Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines.

Authors - Yu Li