ERp19 Polyclonal antibody

ERp19 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20181-1-AP

Synonyms

AGR1, AIF, AIFM1, ERp18, ERp19, hAG 1, PDCD8, PDIA16, TLP19, TXNDC12



Tested Applications

Positive WB detected inhuman brain tissue, human heart tissue, mouse kidney tissue, mouse lung tissue
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20181-1-AP is ERp19 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERp19 fusion protein Ag14045
Full Name thioredoxin domain containing 12 (endoplasmic reticulum)
Calculated molecular weight 172 aa, 19 kDa
Observed molecular weight 20 kDa, 40 kDa
GenBank accession numberBC001493
Gene symbol TXNDC12
Gene ID (NCBI) 51060
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TXNDC12, which is known as thioredoxin domain-containing protein 12, ERp16, ERp18, ERp19, or hTLP19, is a member of the protein disulfide isomerase (PDI) family that plays important roles in cancer development and progression. TXNDC12 has been demonstrated to contribute to tumorigenicity in human gastric cancer by promoting cell growth, migration and invasion(PMID: 25940440). Other study also revealed the overexpression of TXNDC12 promotes HCC metastasis through β-catenin-mediated induction of EMT and that TXNDC12 has the potential to be a prognostic factor and therapeutic target of HCC.(PMID: 31570854)

Protocols

Product Specific Protocols
WB protocol for ERp19 antibody 20181-1-APDownload protocol
IHC protocol for ERp19 antibody 20181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols