Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HUVEC cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human oesophagus cancer tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15508-1-AP targets Transgelin-2-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | transgelin 2 |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | NM_003564 |
| Gene Symbol | Transgelin-2/TAGLN2 |
| Gene ID (NCBI) | 8407 |
| RRID | AB_2199501 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37802 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Transgelin 2 can form multiple complexes, including homodimers and actin complexes. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
| IHC protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
| IP protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
| WB protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TAGLN2 antibody (Cat# 15508-1-AP) has 7 total citations across journals including Cancer Cell International, Molecules, Biosciences Reports, Journal of Gastroenterology and Hepatology, Frontiers in Oncology, PLoS ONE, and Biochemical and Biophysical Research Communications. Research fields span oncology (papillary thyroid carcinoma, gastric cancer, esophageal squamous cell carcinoma, hepatocellular carcinoma), pulmonary fibrosis, and angiogenesis. Applications include WB, IHC, IF, and IP in human samples.
| Species | Application | Title |
|---|---|---|
Cancer Cell Int MiR-613 inhibits the proliferation, migration, and invasion of papillary thyroid carcinoma cells by directly targeting TAGLN2. | ||
Molecules MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway | ||
Biosci Rep The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer. | ||
J Gastroenterol Hepatol The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma. | ||
Front Oncol An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.
| ||
PLoS One Transgelin 2 participates in lovastatin-induced anti-angiogenic effects in endothelial cells through a phosphorylated myosin light chain-related mechanism.
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