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Transgelin-2-specific Polyclonal antibody

Transgelin-2-specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15508-1-AP

Synonyms

TAGLN2, Transgelin 2



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15508-1-AP targets Transgelin-2-specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transgelin 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberNM_003564
Gene symbol TAGLN2
Gene ID (NCBI) 8407
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.

Protocols

Product Specific Protocols
WB protocol for Transgelin-2-specific antibody 15508-1-APDownload protocol
IHC protocol for Transgelin-2-specific antibody 15508-1-APDownload protocol
IF protocol for Transgelin-2-specific antibody 15508-1-APDownload protocol
IP protocol for Transgelin-2-specific antibody 15508-1-APDownload protocol
FC protocol for Transgelin-2-specific antibody 15508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

J Gastroenterol Hepatol

The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma.

Authors - Yan-Yan Du
humanWB,IP

Biochem Biophys Res Commun

Transgelin-2 contributes to proliferation and progression of hepatocellular carcinoma via regulating Annexin A2.

Authors - Jingpei Shi
humanWB,IF

PLoS One

Transgelin 2 participates in lovastatin-induced anti-angiogenic effects in endothelial cells through a phosphorylated myosin light chain-related mechanism.

Authors - Xiao Yuan Y
  • KD Validated