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Transgelin-2-specific Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 15508-1-AP

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Con: 39 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

Transgelin-2-specific Antibody WB HeLa cells 15508-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Transgelin-2-specific Antibody IF HepG2 cells 15508-1-AP

Immunofluorescent analysis of HepG2 cells using 15508-1-AP (Transgelin-2-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Transgelin-2-specific Antibody IHC human kidney tissue 15508-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney using 15508-1-AP(Transgelin-2-specific antibody) at dilution of 1:50 (under 10x lens)

Transgelin-2-specific Antibody IHC human kidney tissue 15508-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney using 15508-1-AP(Transgelin-2-specific antibody) at dilution of 1:50 (under 40x lens)

Transgelin-2-specific Antibody IP L02 cells 15508-1-AP

IP Result of anti-Transgelin-2-specific (IP:15508-1-AP, 3ug; Detection:15508-1-AP 1:500) with L02 cells lysate 1000ug.

Transgelin-2-specific Antibody FC HepG2 cells 15508-1-AP

1X10^6 HepG2 cells were stained with 0.2ug Transgelin-2-specific antibody (15508-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
Human
Positive WB detected in:
HeLa cells, HEK-293 cells
Positive IP detected in:
L02 cells
Positive IHC detected in:
human kidney tissue, human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
transgelin 2
Calculated molecular weight:
22 kDa
Observed molecular weight:
22 kDa
GenBank accession number:
NM_003564
Gene ID (NCBI):
8407
Gene symbol
TAGLN2
Synonyms
TAGLN2, Transgelin 2

Background

The transgelin family is a group of proteins that belong to 22kd actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.


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