CoraLite® Plus 488-conjugated Transketolase Monoclonal antibody
Transketolase Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3E2F3
Cat no : CL488-66016
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL488-66016 is Transketolase Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Transketolase fusion protein Ag16779 |
Full Name | transketolase |
Calculated Molecular Weight | 68 kDa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | BC009970 |
Gene Symbol | TKT |
Gene ID (NCBI) | 7086 |
RRID | AB_2883228 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Transketolase antibody CL488-66016 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |