CoraLite®488-conjugated Transketolase Monoclonal antibody

Transketolase Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3E2F3

Cat no : CL488-66016

Synonyms

FLJ34765, TK, TKT, TKT1, transketolase



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66016-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66016 is Transketolase Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Transketolase fusion protein Ag16779
Full Name transketolase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC009970
Gene symbol TKT
Gene ID (NCBI) 7086
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated Transketolase antibody CL488-66016Download protocol
Standard Protocols
Click here to view our Standard Protocols