Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, human placenta tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16905-1-AP targets UNC13D/Munc13-4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10519 Product name: Recombinant human UNC13D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-327 aa of BC067084 Sequence: MATLLSHPQQRPPFLRQAIKIRRRRVRDLQDPPPQMAPEIQPPSHHFSPEQRALLYEDALYTVLHRLGHPEPNHVTEASELLRYLQEAFHVEPEEHQQTLQRVRELEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGVPGGSPGSRHRQKAVVRHTIPEEETHRTQVITQTLNPVWDETFILEFEDITNASFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPRTETYPDRGQCHLQFQLIHKRRATSASRSQPSYTVHLHLLQQLVSHEVTQHEAGSTSWDGSL Predict reactive species |
| Full Name | unc-13 homolog D (C. elegans) |
| Calculated Molecular Weight | 1090 aa, 103 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC067084 |
| Gene Symbol | UNC13D |
| Gene ID (NCBI) | 201294 |
| RRID | AB_2212713 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q70J99 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UNC13D/Munc13-4 antibody 16905-1-AP | Download protocol |
| IP protocol for UNC13D/Munc13-4 antibody 16905-1-AP | Download protocol |
| WB protocol for UNC13D/Munc13-4 antibody 16905-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UNC13D/Munc13-4 polyclonal antibody (Cat# 16905-1-AP) has 7 citations published in Blood, Molecular Cell, Journal of Experimental Medicine, Journal of Translational Medicine, Cell Reports, Frontiers in Immunology, and Molecular Biology of the Cell. Research fields span immunology (FHL3, T-cell immune synapses, cytotoxic lymphocyte function), cancer biology (pancreatic cancer migration), neuroscience (FMRP mRNA regulation), hematology (platelet exocytosis), and cell biology (Weibel-Palade body secretion).
| Species | Application | Title |
|---|---|---|
Blood Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D. | ||
Mol Cell Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells | ||
J Exp Med Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency. | ||
J Transl Med Recycling machinery of integrin coupled with focal adhesion turnover via RAB11-UNC13D-FAK axis for migration of pancreatic cancer cells | ||
Cell Rep Unveiling the molecular architecture of T cells and immune synapses with cryo-expansion microscopy. | ||
Front Immunol Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets. |
