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  • KD/KO Validated

UNC13D/Munc13-4 Polyclonal antibody

UNC13D/Munc13-4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16905-1-AP

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Munc13-4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D



Tested Applications

Positive WB detected inJurkat cells, human placenta tissue, K-562 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman thymus tissue, human kidney tissue, human lung tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16905-1-AP targets UNC13D/Munc13-4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UNC13D/Munc13-4 fusion protein Ag10519
Full Name unc-13 homolog D (C. elegans)
Calculated molecular weight 1090 aa, 103 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC067084
Gene symbol UNC13D
Gene ID (NCBI) 201294
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UNC13D/Munc13-4 antibody 16905-1-APDownload protocol
IHC protocol for UNC13D/Munc13-4 antibody 16905-1-APDownload protocol
IP protocol for UNC13D/Munc13-4 antibody 16905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Biol Cell

A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells.

Authors - Tarek Chehab
  • KO Validated
humanWB

Front Immunol

Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets.

Authors - Donatella Galgano
humanWB

Blood

Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D.

Authors - Meeths Marie M
humanWB

J Exp Med

Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.

Authors - Frank Cichocki