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  • Featured Product
  • KD/KO Validated

USE1 Polyclonal antibody

USE1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25218-1-AP

Synonyms

MDS032, P31, SLT1, USE1, USE1 like protein, USE1L, Vesicle transport protein USE1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using USE1 Polyclonal antibody (25218-1-AP)

    WB analysis of HEK-293 using 25218-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25218-1-AP (USE1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using USE1 Polyclonal antibody (25218-1-AP)

    WB analysis of Jurkat using 25218-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25218-1-AP (USE1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using USE1 Polyclonal antibody (25218-1-AP)

    IHC staining of human small intestine using 25218-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25218-1-AP (USE1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using USE1 Polyclonal antibody (25218-1-AP)

    IHC staining of human small intestine using 25218-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25218-1-AP (USE1 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25218-1-AP is USE1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USE1 fusion protein Ag18587
Full Name unconventional SNARE in the ER 1 homolog (S. cerevisiae)
Calculated Molecular Weight 259 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC008455
Gene Symbol USE1
Gene ID (NCBI) 55850
RRIDAB_2879966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USE1(Vesicle transport protein USE1) is also named as USE1L, p31 and belongs to the USE1 family. It may be an essential molecule involved in the maintenance of ER morphology (and its deficiency leads to ER stress-induced apoptosis). USE1 also participates in the degradative function of lysosomes. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for USE1 antibody 25218-1-APDownload protocol
IHC protocol for USE1 antibody 25218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation

Authors - Yaqing Liu
  • KD Validated
View Article
humanWB

J Cell Sci

A trap mutant reveals the physiological client spectrum of TRC40.

Authors - Javier Coy-Vergara
View Article