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- KD/KO Validated
VAMP3/Cellubrevin Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 10702-1-AP
CEB, Cellubrevin, SYB3, Synaptobrevin 3, VAMP 3, VAMP3
|Positive WB detected in||HEK-293 cells, MCF-7 cells, mouse brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human heart tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10702-1-AP targets VAMP3/Cellubrevin in WB, IP, IHC, IF, Blocking assay, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VAMP3/Cellubrevin fusion protein Ag1156|
|Full Name||vesicle-associated membrane protein 3 (cellubrevin)|
|Calculated molecular weight||11 kDa|
|Observed molecular weight||11-17 kDa|
|GenBank accession number||BC005941|
|Gene ID (NCBI)||9341|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.
|Product Specific Protocols|
|WB protocol for VAMP3/Cellubrevin antibody 10702-1-AP||Download protocol|
|IHC protocol for VAMP3/Cellubrevin antibody 10702-1-AP||Download protocol|
|IP protocol for VAMP3/Cellubrevin antibody 10702-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Systematic in-depth proteomic analysis of mitochondria-associated endoplasmic reticulum membranes (MAM) in mouse and human testes.
Exogenous H2S Induces Hrd1 S-sulfhydration and Prevents CD36 Translocation via VAMP3 Ubiquitylation in Diabetic Hearts.
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Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.
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CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.
A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis.
KRAS regulation by small non-coding RNAs and SNARE proteins.