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VAMP3/Cellubrevin Antibody

VAMP3/Cellubrevin Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, Blocking assay, ELISA

Conjugate

Unconjugated

Cat no : 10702-1-AP

Synonyms

CEB, Cellubrevin, SYB3, Synaptobrevin 3, VAMP 3, VAMP3



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10702-1-AP targets VAMP3/Cellubrevin in WB, IP, IHC, IF, Blocking assay, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAMP3/Cellubrevin fusion protein Ag1156
Full Name vesicle-associated membrane protein 3 (cellubrevin)
Calculated molecular weight 11 kDa
Observed molecular weight 11-17 kDa
GenBank accession number BC005941
Gene symbol VAMP3
Gene ID (NCBI) 9341
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.

Protocols

Product Specific Protocols
WB protocol for VAMP3/Cellubrevin antibody 10702-1-APDownload protocol
IHC protocol for VAMP3/Cellubrevin antibody 10702-1-APDownload protocol
IP protocol for VAMP3/Cellubrevin antibody 10702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Proteomics

Systematic in-depth proteomic analysis of mitochondria-associated endoplasmic reticulum membranes (MAM) in mouse and human testes.

Authors - Xiaoli Wang
mouseWB,IF

Aging Dis

Exogenous H2S Induces Hrd1 S-sulfhydration and Prevents CD36 Translocation via VAMP3 Ubiquitylation in Diabetic Hearts.

Authors - Miao Yu
humanWB

J Biol Chem

Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.

Authors - Wulin Aerbajinai
humanWB

Br J Pharmacol

CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.

Authors - Fang Bian
humanIHC

Oncotarget

A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis.

Authors - Mitsuhiro Shimura
humanWB,IF

Nat Commun

KRAS regulation by small non-coding RNAs and SNARE proteins.

Authors - Yonglu Che