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  • KD/KO Validated

VAV1 Polyclonal antibody

VAV1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16364-1-AP

Synonyms

Proto oncogene vav, VAV, VAV1



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, HL-60 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human brain tissue, human kidney tissue, human liver tissue, human heart tissue, human spleen tissue, human lung tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16364-1-AP targets VAV1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity gecko, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAV1 fusion protein Ag9049
Full Name vav 1 guanine nucleotide exchange factor
Calculated molecular weight 845 aa, 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC013361
Gene symbol VAV1
Gene ID (NCBI) 7409
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VAV1 antibody 16364-1-APDownload protocol
IHC protocol for VAV1 antibody 16364-1-APDownload protocol
IP protocol for VAV1 antibody 16364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Clin Sci (Lond)

Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13-induced minimal change-like nephropathy.

Authors - Chang-Yien Chan
  • KD Validated
humanWB

Oncotarget

Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity.

Authors - Wei-Zhen Xue
humanWB,IHC

Biochem Biophys Res Commun

Vav1 expression is increased in esophageal squamous cell carcinoma and indicates poor prognosis.

Authors - Xiaolei Zhu
geckoWB,IF

J Biol Chem

Adult astrocytes from reptiles are resistant to proinflammatory activation via sustaining Vav1 expression.

Authors - Nan Du
  • KD Validated