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VCP Antibody

VCP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 10736-1-AP

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP



Tested Applications

Positive WB detected inC6 cells, rat lung tissue, mouse lung tissue, mouse brain tissue, RAW264.7, rat brain tissue, SH-SY5Y cells, human brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, human colon cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10736-1-AP targets VCP in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VCP fusion protein Ag1002
Full Name valosin-containing protein
Calculated molecular weight 89 kDa
Observed molecular weight 90-100 kDa
GenBank accession number BC007562
Gene symbol VCP
Gene ID (NCBI) 7415
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Protocols

Product Specific Protocols
WB protocol for VCP antibody 10736-1-APDownload protocol
IHC protocol for VCP antibody 10736-1-APDownload protocol
IF protocol for VCP antibody 10736-1-APDownload protocol
IP protocol for VCP antibody 10736-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Acupunct Med

Acupuncture regulates the unfolded protein response and inhibits apoptosis in a rat model of heroin relapse.

Authors - Yang Zhang
humanWB,CoIP,IF

Mol Cell Biol

The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.

Authors - Rakesh Ganji
  • KD Validated
humanIHC

Mol Cell Proteomics

Integrative quantitative proteomics unveils proteostasis imbalance in human hepatocellular carcinoma developed on nonfibrotic livers.

Authors - Luc Negroni
humanWB

Hum Mol Genet

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Authors - Zhouteng Tao
mouseWB

Cell Metab

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Authors - Hui Luo