|Positive WB detected in||mouse brain tissue, human heart tissue, mouse heart tissue, rat brain tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human prostate cancer tissue, human prostate tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11663-1-AP targets VDAC2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VDAC2 fusion protein Ag2266|
|Full Name||voltage-dependent anion channel 2|
|Calculated molecular weight||294 aa, 32 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC000165|
|Gene ID (NCBI)||7417|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.
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Identification of sperm head proteins involved in zona pellucida binding.
Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma.