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VEGFR2 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 26415-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

VEGFR2 Antibody IHC human lung cancer tissue 26415-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26415-1-AP (VEGF Receptor 2 antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

VEGFR2 Antibody IHC human lung cancer tissue 26415-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26415-1-AP (VEGF Receptor 2 antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

VEGFR2 Antibody WB human placenta tissue 26415-1-AP

human placenta tissue were subjected to SDS PAGE followed by western blot with 26415-1-AP( VEGF Receptor 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

VEGFR2 Antibody FC HUVEC cells 26415-1-AP

1X10^6 HUVEC cells were stained with 0.2ug VEGF Receptor 2 antibody (26415-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat, sheep
Positive WB detected in:
human placenta tissue, mouse heart tissue
Positive IHC detected in:
human lung cancer tissue, human oesophagus cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
HUVEC cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:200-1:800
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
VEGFR2 fusion protein Ag24589
Full name:
kinase insert domain receptor (a type III receptor tyrosine kinase)
Calculated molecular weight:
1356aa,152 kDa
Observed molecular weight:
200 kDa
GenBank accession number:
BC131822
Gene ID (NCBI):
3791
Gene symbol
KDR
Synonyms
CD309, Fetal liver kinase 1, FLK 1, FLK1, KDR, Kinase insert domain receptor, VEGF Receptor 2, VEGFR, VEGFR 2, VEGFR2

Background

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

VEGFR2 Rabbit Polyclonal antibody

Kyosuke (Verified Customer), 06-12-2019
Applications
Western Blot,
Primary Antibody Dilution
1/1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
HUVECs
Lot Number
I am working on angiogenesis study and use this for WB on HUVECs. It works very well.

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