|Positive IHC detected in||mouse brain tissue, human testis tissue, human heart tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12006-1-AP targets VPS45 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPS45 fusion protein Ag2658|
|Full Name||vacuolar protein sorting 45 homolog (S. cerevisiae)|
|Calculated molecular weight||570aa,65 kDa|
|Observed molecular weight|
|GenBank accession number||BC012932|
|Gene ID (NCBI)||11311|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)
Rice protein suppresses ROS generation and stimulates antioxidant gene expression via Nrf2 activation in adult rats.
J Cell Sci
The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jerome (Verified Customer) (05-28-2020)
Very inconclusive, a lot of background.