VPS45 Polyclonal antibody

VPS45 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12006-1-AP

Synonyms

h VPS45, H1, H1VPS45, hlVps45, VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A



Tested Applications

Positive IHC detected inmouse brain tissue, human testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12006-1-AP targets VPS45 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS45 fusion protein Ag2658
Full Name vacuolar protein sorting 45 homolog (S. cerevisiae)
Calculated molecular weight 570 aa, 65 kDa
GenBank accession numberBC012932
Gene symbol VPS45
Gene ID (NCBI) 11311
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

Protocols

Product Specific Protocols
WB protocol for VPS45 antibody 12006-1-APDownload protocol
IHC protocol for VPS45 antibody 12006-1-APDownload protocol
IF protocol for VPS45 antibody 12006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Gene

Rice protein suppresses ROS generation and stimulates antioxidant gene expression via Nrf2 activation in adult rats.

Authors - Hui Li
humanWB

J Cell Sci

The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.

Authors - Laufman Orly O

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jerome (Verified Customer) (05-28-2020)

Very inconclusive, a lot of background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 100
  • Cell Tissue Type: HeLa
VPS45 Antibody Immunofluorescence validation (100 dilution) in HeLa (Cat no:12006-1-AP)