VPS45 Antibody

VPS45 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Publications

0

Cat no : 12616-1-AP

Synonyms

h VPS45, H1, H1VPS45, hlVps45, VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A



Tested Applications

Positive WB detected inmouse liver tissue, rat pancreas tissue, human kidney tissue, mouse brain tissue
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12616-1-AP targets VPS45 in IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS45 fusion protein Ag3289
Full Name vacuolar protein sorting 45 homolog (S. cerevisiae)
Calculated molecular weight 570aa,65 kDa
Observed molecular weight 65-70 kDa
GenBank accession number BC028382
Gene symbol VPS45
Gene ID (NCBI) 11311
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

Protocols

Product Specific Protocols
WB protocol for VPS45 antibody 12616-1-APDownload protocol
IP protocol for VPS45 antibody 12616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols