Vimentin Antibody 2 Publications

Mouse Monoclonal| Catalog number: 60330-1-Ig |CloneNo.: 3H9D1

Featured Product KD/KO validated

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Con: 111 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
Jurkat cells, A549 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, Raji cells, ROS1728 cells

Positive IHC detected in:
human renal cell carcinoma tissue

Positive IF detected in:
HeLa cells

Positive FC detected in:
Jurkat cells

Recommended dilution:
WB : 1:1000-1:8000
IHC : 1:20-1:2000
IF : 1:20-1:200

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
vimentin

Calculated molecular weight:
466aa,54 kDa

Observed molecular weight:
55-60 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
VIM

Synonyms
FLJ36605, VIM, Vimentin
Background

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.


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