Vinculin Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 66305-1-Ig
CMD1W, Metavinculin, MVCL, VCL, Vinculin
|Positive WB detected in||HeLa cells, pig heart tissue, rat heart tissue|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66305-1-Ig targets Vinculin in FC, IF, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Reactivity||human, mouse, rat, pig|
|Cited Species||hamster, human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Vinculin fusion protein Ag24946|
|Calculated molecular weight||1133aa,124 kDa|
|Observed molecular weight||117 kDa|
|GenBank accession number||BC039174|
|Gene ID (NCBI)||7414|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
|Product Specific Protocols|
|WB protocol for Vinculin antibody 66305-1-Ig||Download protocol|
|IHC protocol for Vinculin antibody 66305-1-Ig||Download protocol|
|IF protocol for Vinculin antibody 66305-1-Ig||Download protocol|
|FC protocol for Vinculin antibody 66305-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
J Mol Neurosci
The Mechanism of Rap1 Regulates N-cadherin to Control Neuronal Migration.
Nucleic Acids Res
BRCA1-associated R-loop affects transcription and differentiation in breast luminal epithelial cells.
Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer.
Can J Physiol Pharmacol
Focal adhesions are involved in simulated microgravity-induced basilar and femoral arterial remodelling in rats.
The impact of photofunctionalized gold nanoparticles on osseointegration.
Cancer Biol Ther
CRMP4a suppresses cell motility by sequestering RhoA activity in prostate cancer cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Huai-Chin Chiang (06-08-2019)
Serving as a loading control, this antibody is not that good compare to other. Still work to some extent.