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XIAP Polyclonal antibody

XIAP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10037-1-Ig

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2



Tested Applications

Positive WB detected inRAW 264.7 cells, Jurkat cells, HepG2 cells, HeLa cells, COLO 320 cells, C6 cells, NIH/3T3 cells, Raji cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10037-1-Ig targets XIAP in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XIAP fusion protein Ag0022
Full Name X-linked inhibitor of apoptosis
Calculated molecular weight 60 kDa
Observed molecular weight 45-57 kDa
GenBank accession numberBC032729
Gene symbol XIAP
Gene ID (NCBI) 331
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56 kDa.

Protocols

Product Specific Protocols
WB protocol for XIAP antibody 10037-1-IgDownload protocol
IHC protocol for XIAP antibody 10037-1-IgDownload protocol
IF protocol for XIAP antibody 10037-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.

Authors - Weimei Tang
humanWB

Int J Nanomedicine

Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.

Authors - Muhammad Waseem Khan
humanWB

J Agric Food Chem

β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.

Authors - Heshan Xu
humanWB

Exp Ther Med

PCSK9 regulates apoptosis in human lung adenocarcinoma A549 cells via endoplasmic reticulum stress and mitochondrial signaling pathways.

Authors - Xiaohui Xu
humanWB

Oncol Lett

Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway.

Authors - Hongtao Duan
humanWB

Onco Targets Ther

Brevilin A promotes oxidative stress and induces mitochondrial apoptosis in U87 glioblastoma cells.

Authors - Jie Wang