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YTHDF1 Polyclonal antibody
YTHDF1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, RIP, IP, IHC, IF, CoIP,ELISA
Conjugate
Unconjugated
Cat no : 17479-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, HepG2 cells, Jurkat cells, A549 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue, human kidney tissue, human testis tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 29 publications below |
| WB | See 55 publications below |
| IHC | See 8 publications below |
| IF | See 12 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
| RIP | See 11 publications below |
Product Information
17479-1-AP targets YTHDF1 in WB, RIP, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | YTHDF1 fusion protein Ag11509 |
| Full Name | YTH domain family, member 1 |
| Calculated molecular weight | 559 aa, 61 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC050284 |
| Gene symbol | YTHDF1 |
| Gene ID (NCBI) | 54915 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDF1 antibody 17479-1-AP | Download protocol |
| IHC protocol for YTHDF1 antibody 17479-1-AP | Download protocol |
| IF protocol for YTHDF1 antibody 17479-1-AP | Download protocol |
| IP protocol for YTHDF1 antibody 17479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.
| ||
Nucleic Acids Res The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression.
| ||
Cell Death Dis m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner. | ||
Oncogene Dynamic m6A mRNA methylation reveals the role of METTL3-m6A-CDCP1 signaling axis in chemical carcinogenesis.
| ||
Int J Obes (Lond) mRNA m6A plays opposite role in regulating UCP2 and PNPLA2 protein expression in adipocytes. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sarah (Verified Customer) (01-05-2021) | Works great in WB at 1/1000 (5% Milk TBS Tween). Tested on HeLa and 293T lysates;
|
FH Hannah (Verified Customer) (08-14-2020) | Band observed by WB of correct size that is reduced with siRNA knockdown but required long exposure or Pico supersignal ECL for a good signal from fibroblast lysates
|
FH Biao (Verified Customer) (03-11-2020) | This antibody is very specific and good quality.
|
FH Zizhao (Verified Customer) (12-03-2018) |
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