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  • KD/KO Validated

YTHDF1 Polyclonal antibody

YTHDF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 17479-1-AP

Synonyms

C20orf21, DACA 1, YTH domain family protein 1, YTH domain family, member 1, YTHDF1



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells, Jurkat cells, A549 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human kidney tissue, human testis tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17479-1-AP targets YTHDF1 in WB, RIP, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF1 fusion protein Ag11509
Full Name YTH domain family, member 1
Calculated molecular weight 559 aa, 61 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC050284
Gene symbol YTHDF1
Gene ID (NCBI) 54915
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.

Protocols

Product Specific Protocols
WB protocol for YTHDF1 antibody 17479-1-APDownload protocol
IHC protocol for YTHDF1 antibody 17479-1-APDownload protocol
IF protocol for YTHDF1 antibody 17479-1-APDownload protocol
IP protocol for YTHDF1 antibody 17479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Nature

Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.

Authors - Dali Han
  • KO Validated
mouseWB,IF

Nucleic Acids Res

The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression.

Authors - Mengru Zhuang
  • KO Validated
mouseWB

Cell Death Dis

m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.

Authors - Ruifan Wu
humanWB

Oncogene

Dynamic m6A mRNA methylation reveals the role of METTL3-m6A-CDCP1 signaling axis in chemical carcinogenesis.

Authors - Fan Yang
  • KD Validated
mouseWB

Int J Obes (Lond)

mRNA m6A plays opposite role in regulating UCP2 and PNPLA2 protein expression in adipocytes.

Authors - Xinxia Wang
humanWB

Genes Dev

PMID: 30463905

Authors - Rosa M Rubio

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah (Verified Customer) (01-05-2021)

Works great in WB at 1/1000 (5% Milk TBS Tween). Tested on HeLa and 293T lysates;

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa and HEL293T
FH

Hannah (Verified Customer) (08-14-2020)

Band observed by WB of correct size that is reduced with siRNA knockdown but required long exposure or Pico supersignal ECL for a good signal from fibroblast lysates

  • Applications: Western Blot,Immunofluorescence
  • Primary Antibody Dilution: 1/1000 for WB; 1/100 for IF
  • Cell Tissue Type: human fibroblasts
FH

Biao (Verified Customer) (03-11-2020)

This antibody is very specific and good quality.

  • Applications: Western Blot,
  • Cell Tissue Type: hct-116
FH

Zizhao (Verified Customer) (12-03-2018)

Great Antibody!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HaCaT/human keratinocytes
YTHDF1 Antibody Western Blot, validation (1:1000 dilution) in HaCaT/human keratinocytes (Cat no:17479-1-AP)