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ZAP70 Polyclonal antibody

ZAP70 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22207-1-AP

Synonyms

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Polyclonal antibody (22207-1-AP)

    WB analysis of Jurkat using 22207-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22207-1-AP (ZAP70 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using ZAP70 Polyclonal antibody (22207-1-AP)

    WB analysis of RAW264.7 using 22207-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22207-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using ZAP70 Polyclonal antibody (22207-1-AP)

    IP experiment of Jurkat using 22207-1-AP

    IP result of anti-ZAP70 (IP:22207-1-AP, 4ug; Detection:22207-1-AP 1:1000) with Jurkat cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Polyclonal antibody (22207-1-AP)

    IHC staining of human lymphoma using 22207-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 22207-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Polyclonal antibody (22207-1-AP)

    IHC staining of human lymphoma using 22207-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 22207-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using ZAP70 Polyclonal antibody (22207-1-AP)

    IF Staining of RAW 264.7 using 22207-1-AP

    Immunofluorescent analysis of RAW 264.7 cells using 22207-1-AP (ZAP70 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells, RAW264.7
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22207-1-AP is ZAP70 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag17527
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated Molecular Weight 619 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
RRIDAB_2879029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZAP70 antibody 22207-1-APDownload protocol
IHC protocol for ZAP70 antibody 22207-1-APDownload protocol
IF protocol for ZAP70 antibody 22207-1-APDownload protocol
IP protocol for ZAP70 antibody 22207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols