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  • Featured Product
  • KD/KO Validated

ZC3HAV1 Monoclonal antibody

ZC3HAV1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G10B9

Cat no : 66413-1-Ig

Synonyms

FLB6421, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    WB analysis of HEK-293 using 66413-1-Ig

    WB result of ZC3HAV1 antibody (66413-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3HAV1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of Jurkat cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    WB analysis of Jurkat using 66413-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    WB analysis of HeLa using 66413-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    WB analysis of HEK-293 using 66413-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    IHC staining of human kidney using 66413-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    IHC staining of human kidney using 66413-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    IF Staining of HepG2 using 66413-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66413-1-Ig(ZC3HAV1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66413-1-Ig targets ZC3HAV1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZC3HAV1 fusion protein Ag10364
Full Name zinc finger CCCH-type, antiviral 1
Calculated Molecular Weight699aa,78 kDa; 902aa,101 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC025308
Gene Symbol ZC3HAV1
Gene ID (NCBI) 56829
RRIDAB_2881785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.

Protocols

Product Specific Protocols
WB protocol for ZC3HAV1 antibody 66413-1-IgDownload protocol
IHC protocol for ZC3HAV1 antibody 66413-1-IgDownload protocol
IF protocol for ZC3HAV1 antibody 66413-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

mBio

The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts.

Authors - Ana Cristina Gonzalez-Perez
  • KO Validated
View Article
humanIF

J Virol

Riplet Binds the Zinc Finger Antiviral Protein (ZAP) and Augments ZAP-Mediated Restriction of HIV-1.

Authors - Marlene V Buckmaster
View Article
humanWB

EMBO Rep

Interferon inhibits a model RNA virus via a limited set of inducible effector genes

Authors - Matthew B McDougal
View Article