ZC3HAV1 Monoclonal antibody

ZC3HAV1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G10B9

Cat no : 66413-1-Ig

Synonyms

FLB6421, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66413-1-Ig targets ZC3HAV1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZC3HAV1 fusion protein Ag10364
Full Name zinc finger CCCH-type, antiviral 1
Calculated molecular weight699aa,78 kDa; 902aa,101 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC025308
Gene symbol ZC3HAV1
Gene ID (NCBI) 56829
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.

Protocols

Product Specific Protocols
WB protocol for ZC3HAV1 antibody 66413-1-IgDownload protocol
IHC protocol for ZC3HAV1 antibody 66413-1-IgDownload protocol
IF protocol for ZC3HAV1 antibody 66413-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols