|Positive WB detected in||mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells|
|Positive IP detected in||mouse testis tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
12680-1-AP targets ZFYVE27 in WB, IP-MS, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ZFYVE27 fusion protein Ag3369|
|Full Name||zinc finger, FYVE domain containing 27|
|Calculated molecular weight||411 aa, 46 kDa|
|Observed molecular weight||46-50 kDa|
|GenBank accession number||BC030621|
|Gene ID (NCBI)||118813|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ZFYVE27, also named as Protrudin, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. ZFYVE27 localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. ZFYVE27 is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.
Cell Death Dis
Protrudin modulates seizure activity through GABAA receptor regulation.
J Biol Chem
Protrudin Regulates Endoplasmic Reticulum Morphology and Function Associated with the Pathogenesis of Hereditary Spastic Paraplegia.
Fatty acid synthase cooperates with protrudin to facilitate membrane outgrowth of cellular protrusions.
Cell Mol Life Sci
ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.
Proc Natl Acad Sci U S A
Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.