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  • KD/KO Validated

ZFYVE27 Polyclonal antibody

ZFYVE27 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP-MS, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12680-1-AP

Synonyms

PROTRUDIN, RP11 459F3.2, SPG33, ZFYVE27



Tested Applications

Positive WB detected inmouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12680-1-AP targets ZFYVE27 in WB, IP-MS, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFYVE27 fusion protein Ag3369
Full Name zinc finger, FYVE domain containing 27
Calculated molecular weight 411 aa, 46 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC030621
Gene symbol ZFYVE27
Gene ID (NCBI) 118813
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZFYVE27, also named as Protrudin, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. ZFYVE27 localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. ZFYVE27 is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.

Protocols

Product Specific Protocols
WB protocol for ZFYVE27 antibody 12680-1-APDownload protocol
IP protocol for ZFYVE27 antibody 12680-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,CoIP

Cell Death Dis

Protrudin modulates seizure activity through GABAA receptor regulation.

Authors - Xi Lu
humanWB

J Biol Chem

Protrudin Regulates Endoplasmic Reticulum Morphology and Function Associated with the Pathogenesis of Hereditary Spastic Paraplegia.

Authors - Yutaka Hashimoto
humanWB

Sci Rep

Fatty acid synthase cooperates with protrudin to facilitate membrane outgrowth of cellular protrusions.

Authors - Chuanling Zhang
  • KD Validated
human

Cell Mol Life Sci

ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.

Authors - James W Connell
humanWB,IF,IP

Proc Natl Acad Sci U S A

Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.

Authors - Chang Jaerak J
  • KD Validated
humanWB

Nat Commun

Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.

Authors - Parikshit Bagchi