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  • Western Blot (WB) analysis of various lysates using ZNF238/ZBTB18 Recombinant antibody (86544-3-RR)

    WB analysis using 86544-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86544-3-RR (ZNF238 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86544-3-RR

    Affinity and Kinetic Characterization of 86544-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86544-3-RR against Human ZNF238/ZBTB18 were performed. The affinity constant is below 1 pM.

ZNF238/ZBTB18 Recombinant antibody

ZNF238/ZBTB18 Recombinant Antibody for WB, ELISA

Cat No. 86544-3-RR
Clone No.251389B9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ZNF238, 58 kDa repressor protein, C2H2 171, RP58, TAZ 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86544-3-RR targets ZNF238/ZBTB18 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZNF238/ZBTB18 fusion protein Ag3406 Predict reactive species
Full Name zinc finger protein 238
Calculated Molecular Weight 531 aa, 59 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC036677
Gene Symbol ZNF238/ZBTB18
Gene ID (NCBI) 10472
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ99592
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF238 is a member of the BTB/POZ-ZF protein family, which involve in development and cancer formation, for example BCL-6, PLZF, and HIC-1. It's a transcriptional repressor involve in myogenesis and brain development. By directly repressing the expression of two skeletal myogenesis inhibitors, ID2 and ID3, ZNF238 plays a key role in myogenesis. It can control cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. Besides, ZNF238 involves in the organization of nuclear chromosomes, for its specific binding to the consensus DNA sequence that contains the E box core, and recruiting chromatin remodeling multi-protein complex. ZNF238 proteins has apparent molecular masses of 60 and 48 kD. Specific binding is found for a 60-kDa band which corresponds to the full length of RP58 protein. In addition, a 48-kDa band, thought to be the truncated form 2 is detected (PMID: 9756912).

Protocols

Product Specific Protocols
WB protocol for ZNF238/ZBTB18 antibody 86544-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols