AMACR/p504S Antibody

AMACR/p504S Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15918-1-AP

Synonyms

2 methylacyl CoA racemase, alpha methylacyl CoA racemase, AMACR, CBAS4, p504S, RACE



Tested Applications

Positive WB detected inmulti-cells, DU 145 cells, HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The 15918-1-AP immunogen is Fusion Protein AMACR/p504S protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMACR/p504S fusion protein Ag8720
Full Name alpha-methylacyl-CoA racemase
Calculated molecular weight 382aa,42 kDa
Observed molecular weight 42 kDa
GenBank accession number BC009471
Gene symbol AMACR
Gene ID (NCBI) 23600
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMACR(Alpha-methylacyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing ang it can form a a dimer of 70-75 kDa(PMID:21812041). Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

Protocols

Product Specific Protocols
WB protocol for AMACR/p504S antibody 15918-1-APDownload protocol
IHC protocol for AMACR/p504S antibody 15918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

The auxiliary diagnostic value of prostate-specific antigen and α-methylacyl-CoA racemase in prostate cancer.

Authors - Daijun Yang