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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human FLT3 Ligand

Activity

Typically ≤ 0.8 ng/mL EC50

Species Reactivity

Human

Purity

>95%

Cat no : HZ-1151

Synonyms

Fms-related tyrosine kinase 3 ligand Fms-related tyrosine kinase3 ligand, Fms-related tyrosine kinase-3 ligand



Technical Specifications

GeneID 2323
Species Human
Expression HEK293
Activity Typically ≤ 0.8 ng/mL EC50
Purity >95%
Molecular Mass 24 to 30 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA)

Product Description

Animal-free Recombinant Human FLT3 Ligand is expressed from human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 24 to 30 kDa. This cytokine is produced in a serum-free, chemically defined media. The molecular mass is a narrower range than reported when this cytokine is expressed in insect Sf21 cells (17 to 30 kDa), emphasizing the difference in glycosylation in different expression systems. FLT3 Ligand is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation.

Background

FMS-related tyrosine kinase 3 ligand (FLT3 Ligand) is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation (PMID PMID: 8590775; 7544638; 12176867).

Synonyms

Fms-related tyrosine kinase 3 ligand Fms-related tyrosine kinase3 ligand, Fms-related tyrosine kinase-3 ligand

Publications

SpeciesTitle
human

Haematologica

CD123 expression patterns and selective targeting with a CD123-specific antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia.

Authors - Evgeniya Angelova
human

Blood Adv

A CD123-targeting antibody-drug conjugate, IMGN632, designed to eradicate AML while sparing normal bone marrow cells.

Authors - Yelena Kovtun
human

Blood Res

Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement-free monolayer culture method.

Authors - So-JungKim
human

PLoS One

A synthetic model of human beta-thalassemia erythropoiesis using CD34+ cells from healthy adult donors.

Authors - Y TerryLee
human

Immunol Cell Biol

Myeloid-derived suppressor cells can be efficiently generated from human hematopoietic progenitors and peripheral blood monocytes.

Authors - SílviaCasacuberta-Serra
human

J Tissue Eng Regen Med

Iron dose-dependent differentiation and enucleation of human erythroblasts in serum-free medium.

Authors - ColleenByrnes