• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN alpha 2B protein

Activity

Typically ≤ 0.12 ng/mL EC50

Specific Activity

9.6x10^7 IU/mg calibrated against IFN alpha 2B reference standard (NIBSC cat no: 95/656). Reference use only. Specific activity is not routinely tested

Species Reactivity

human

Purity

>95%

Cat no : HZ-1072

Synonyms

IFN alpha 2, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A



Technical Specifications

GeneID 3440
Species Human
Expression HEK293
Activity Typically ≤ 0.12 ng/mL EC50
Specific Activity9.6x10^7 IU/mg calibrated against IFN alpha 2B reference standard (NIBSC cat no: 95/656). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01563
Molecular Mass 16 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interferon alpha 2 is a type 1 interferon secreted by virus-infected cells to protect surrounding cells from the pathogen. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761)

Synonyms

IFN alpha 2, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A

Publications

SpeciesTitle
human

J Virol Methods

Screening of novel drugs for inhibiting hepatitis E virus replication.

Authors - Takashi Nishiyama

Antiviral Res

Antiviral candidates against the hepatitis E virus (HEV) and their combinations inhibit HEV growth in in vitro.

Authors - Takashi Nishiyama
human

Cancer Sci

Combination of tumor necrosis factor-α and epidermal growth factor induces the adrenergic-to-mesenchymal transdifferentiation in SH-SY5Y neuroblastoma cells.

Authors - Yue Huang