• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN beta protein

Activity

0.015-0.08 ng/mL EC50

Specific Activity

1.56x10^9 IU/mg calibrated against IFN beta reference standard (NIBSC cat no: 00/572). Reference use only. Specific activity is not routinely tested

Species Reactivity

human

Purity

>95%

Cat no : HZ-1298

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast



Technical Specifications

GeneID 3456
Species Human
Expression HEK293
Activity 0.015-0.08 ng/mL EC50
Specific Activity1.56x10^9 IU/mg calibrated against IFN beta reference standard (NIBSC cat no: 00/572). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01574
Molecular Mass 18 to 22 kDa, glycosylated
Formulation Sodium Acetate pH 4.8 + 150mM NaCl + CHAPS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water.

Background

IFN beta is a member of type I family of interferons. It binds to the IFNα/β receptor (IFNAR), activating the Jak/ STAT pathway. Activation of this signaling pathway results in upregulating genes that inhibit viral infection and regulate MHC class I antigens. It is primarily produced by fibroblasts and monocytes. In addition to inhibiting viral infection, IFN beta is also involved in regulating and activating immune response against bacteria, parasite and tumor cells. Multiple sclerosis is characterized by a deficiency of IFN beta 1. An injectable form of IFN beta 1 is used for MS treatment (PMID: 22222875; 29258190; 8602746; 8469318).

Synonyms

Fibroblast interferon, IFB, IFF, IFN beta, IFNB, IFNB1, Interferon beta, interferon, beta 1, fibroblast

Publications

SpeciesTitle
human

Cell Rep

Critical role of Syk-dependent STAT1 activation in innate antiviral immunity.

Authors - Shasha Liu
human

J Innate Immun

Positive Feedback Loop of Long Noncoding RNA OASL-IT1 and Innate Immune Response Restricts the Replication of Zika Virus in Epithelial A549 Cells.

Authors - Yi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Janina (Verified Customer) (03-28-2022)

I asked the students who worked with it and they think it works fine. No bad surprises but also nothing special happen.

  • Applications: Cell culture
FH

Christine (Verified Customer) (12-14-2021)

I induced the expression of interferon responsive genes by treating HEK293T cells for 24 or 48h with various concentrations of IFN beta. Samples were then analysed by western blot for the detection of an IFN induced protein, which was absent at t=0, clearly induced at 24h and even better expressed at 48h. The lowest amount of IFN analysed here was 3 ng/ml. I also tried IFN alpha 2a (cat number HZ-1066) at the same time and the results were also very good, identical.

  • Applications: Cell culture
  • Cell Tissue Type: HEK293T