- HEK293 expressed
- Endotoxin-free
- Animal-component free
Animal-free Recombinant Human SCF
Cat no : HZ-1024
Synonyms
c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor
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Technical Specifications
| GeneID | 4254 |
| Species | human |
| Expression | HEK293 |
| Activity | Typically ≤ 25 ng/mL EC50 |
| Purity | >95% |
| Molecular Mass | 35 to 45 kDa, monomer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human |
Stability and Reconstitution
| Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Product Description
Animal-free Recombinant Human SCF is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 35 to 45 kDa. This product is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media and other applications. SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages.
Background
SCF is a hematopoietic growth factor that works by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells (PMID: 12773427)
Synonyms
c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor
Publications
| Species | Title |
|---|---|
Haematologica CD123 expression patterns and selective targeting with a CD123-specific antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia. | |
Blood Res Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement-free monolayer culture method. | |
PLoS One A synthetic model of human beta-thalassemia erythropoiesis using CD34+ cells from healthy adult donors. | |
J Tissue Eng Regen Med Iron dose-dependent differentiation and enucleation of human erythroblasts in serum-free medium. | |
Oncotarget Sensitizing leukemia stem cells to NF-κB inhibitor treatment in vivo by inactivation of both TNF and IL-1 signaling. | |
Stem Cells Brief report: efficient generation of hematopoietic precursors and progenitors from human pluripotent stem cell lines. |
