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Animal-free Recombinant Human SCF
Humankine®9 Publications

Catalog number: HZ-1024

HEK293 expressed Endotoxin-free Animal-component free

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Freight/Packing £00.00

Format: Lyophilized

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Technical Specifications

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SCF activity assay

The activity was determined by the dose­-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

SCF purity

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
4254
Species:
Human
Expression:
HEK293
Activity:
Typically ≤ 25 ng/mL EC50
Purity:
>95%
Molecular Mass:
35 to 45 kDa, monomer, glycosylated
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Product Description:

Animal-free Recombinant Human SCF is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 35 to 45 kDa. This product is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media and other applications. SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages.



Background:

SCF is a hematopoietic growth factor that works by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells (PMID: 12773427)



Synonyms:
Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor cKit Ligand, c Kit Ligand



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