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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human SCF protein

Activity

Typically ≤ 25 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1024

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

Product data gallery View All

  • SCF activity assay

    The activity was determined by the dose­-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

  • SCF activity assay

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Technical Specifications

GeneID 4254
Species human
Expression HEK293
Activity Typically ≤ 25 ng/mL EC50
Purity >95%
Molecular Mass 35 to 45 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

SCF is a hematopoietic growth factor that works by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells (PMID: 12773427)

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

Publications

SpeciesTitle
human

Haematologica

CD123 expression patterns and selective targeting with a CD123-specific antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia.

Authors - Evgeniya Angelova
View Article
human

Blood Res

Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement-free monolayer culture method.

Authors - So-JungKim
View Article
human

PLoS One

A synthetic model of human beta-thalassemia erythropoiesis using CD34+ cells from healthy adult donors.

Authors - Y TerryLee
View Article
human

Sci Rep

A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro.

Authors - Dezhi Zhou
View Article
human

J Tissue Eng Regen Med

Iron dose-dependent differentiation and enucleation of human erythroblasts in serum-free medium.

Authors - ColleenByrnes
View Article
human

Stem Cell Res Ther

Derivation and propagation of spermatogonial stem cells from human pluripotent cells.

Authors - Huiming Xu
View Article