Alpha Tubulin Monoclonal antibody
Alpha Tubulin Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, Canine and More (5)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
293
CloneNo.
1E4C11
Cat no : 66031-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, multi-cells, SW 1990 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, rat brain tissue |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 253 publications below |
| IF | See 21 publications below |
Product Information
The immunogen of 66031-1-Ig is Alpha Tubulin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat, Canine |
| Cited Reactivity | Bovine, Drosophila, human, mouse, rat, rice, silkworm, tick |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Alpha Tubulin fusion protein Ag18034 |
| Full Name | tubulin, alpha 1b |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC009314 |
| Gene symbol | TUBA1B |
| Gene ID (NCBI) | 10376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
What is the function of alpha tubulin?
Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.
Where is alpha-tubulin expressed?
Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.
What are the post-translational modifications of alpha tubulin?
The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3 Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).
1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).
2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).
3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha Tubulin antibody 66031-1-Ig | Download protocol |
| IHC protocol for Alpha Tubulin antibody 66031-1-Ig | Download protocol |
| IF protocol for Alpha Tubulin antibody 66031-1-Ig | Download protocol |
| IP protocol for Alpha Tubulin antibody 66031-1-Ig | Download protocol |
| FC protocol for Alpha Tubulin antibody 66031-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol β-TrCP Restricts Lipopolysaccharide (LPS)-Induced Activation of TRAF6-IKK Pathway Upstream of IκBα Signaling. | ||
Hum Mol Genet Nearly complete deletion of BubR1 causes microcephaly through shortened mitosis and massive cell death. | ||
Dev Cell Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation. | ||
J Biol Chem Legionella effector RavD binds phosphatidylinositol-3-phosphate and helps suppress endolysosomal maturation of the Legionella-containing vacuole. | ||
Neurol Res Neuroprotective effects of different frequency preconditioning exercise on neuronal apoptosis after focal brain ischemia in rats. | ||
Int J Biochem Cell Biol Glutamate affects the CYP1B1- and CYP2U1-mediated hydroxylation of arachidonic acid metabolism via astrocytic mGlu5 receptor. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Azita (Verified Customer) (06-02-2021) | Immunohistochemistry labelling of (4% PFA) fixed mice spinal cord tissues using Alpha Tubulin Monoclonal Antibody at dilution of 1:50 showed strong labelling.
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FH Scooby (Verified Customer) (10-05-2020) | happy
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FH Lauren (Verified Customer) (10-05-2020) | thanks
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FH Chun (Verified Customer) (12-05-2019) | An excellent antibody
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