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alpha Tubulin Antibody 111 Publications

Mouse Monoclonal| Catalog number: 66031-1-Ig |CloneNo.: 1E4C11

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Con: 260 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

alpha Tubulin Antibody WB multi-cells 66031-1-Ig

Western blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody 66031-1-Ig at a dilution of 1:10000.

alpha Tubulin Antibody WB HeLa cells 66031-1-Ig

Western blot of Hela cell with anti-Tubulin-Alpha (66031-1-Ig) at various dilutions.

alpha Tubulin Antibody IF HepG2 cells 66031-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

alpha Tubulin Antibody IP HeLa cells 66031-1-Ig

IP Result of anti-alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:66031-1-Ig 1:1000) with HeLa cells lysate 2800ug.

alpha Tubulin Antibody IF HepG2 cells 66031-1-Ig

Immunofluorescent analysis of HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

alpha Tubulin Antibody IHC human tonsillitis tissue 66031-1-Ig

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig( alpha Tubulin antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

alpha Tubulin Antibody IHC human tonsillitis tissue 66031-1-Ig

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig( alpha Tubulin antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

alpha Tubulin Antibody FC HeLa cells 66031-1-Ig

1X10^6 HeLa cells were stained with 0.2ug alpha Tubulin antibody (66031-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

Species specificity:
human, mouse, rat, dog, rice, silkworm
Positive WB detected in:
multi-cells, HeLa
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human tonsillitis tissue, human liver cancer tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, MCF-7 cells, Hela cells, MDCK cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
alpha Tubulin fusion protein Ag18034
Full name:
tubulin, alpha 1b
Calculated molecular weight:
50 kDa
Observed molecular weight:
52 kDa
GenBank accession number:
BC009314
Gene ID (NCBI):
10376
Gene symbol
TUBA1B
Synonyms
alpha Tubulin, Alpha tubulin ubiquitous, TUBA1B, tubulin, Tubulin alpha, tubulin, alpha 1b

Background

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251–64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528–R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94–101 (2011).



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