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alpha Tubulin Antibody 85 Publications

Mouse Monoclonal| Catalog number: 66031-1-Ig |CloneNo.: 1E4C11

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Con: 260 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

alpha Tubulin Antibody WB multi-cells 66031-1-Ig

Western blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody 66031-1-Ig at a dilution of 1:10000.

alpha Tubulin Antibody WB HeLa cells 66031-1-Ig

Western blot of Hela cell with anti-Tubulin-Alpha (66031-1-Ig) at various dilutions.

alpha Tubulin Antibody IF HepG2 cells 66031-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

alpha Tubulin Antibody IP HeLa cells 66031-1-Ig

IP Result of anti-alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:66031-1-Ig 1:1000) with HeLa cells lysate 2800ug.

alpha Tubulin Antibody IF HepG2 cells 66031-1-Ig

Immunofluorescent analysis of HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

alpha Tubulin Antibody IHC human tonsillitis tissue 66031-1-Ig

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig( alpha Tubulin antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

alpha Tubulin Antibody IHC human tonsillitis tissue 66031-1-Ig

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig( alpha Tubulin antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

alpha Tubulin Antibody FC HeLa cells 66031-1-Ig

1X10^6 HeLa cells were stained with 0.2ug alpha Tubulin antibody (66031-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

Species specificity:
human, mouse, rat, dog, rice, silkworm
Positive WB detected in:
multi-cells, HeLa
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human tonsillitis tissue, human liver cancer tissue, human testis tissue
Positive IF detected in:
HepG2 cells, MCF-7 cells, Hela cells, MDCK cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
alpha Tubulin fusion protein Ag18034
Full name:
tubulin, alpha 1b
Calculated molecular weight:
50 kDa
Observed molecular weight:
52 kDa
GenBank accession number:
BC009314
Gene ID (NCBI):
10376
Gene symbol
TUBA1B
Synonyms
alpha Tubulin, Alpha tubulin ubiquitous, TUBA1B, tubulin, Tubulin alpha, tubulin, alpha 1b

Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This antibody specifically recognizes Tubulin Alpha.


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