Tested Applications
| Positive WB detected in | L02 cells, human testis tissue, Jurkat cells, MCF-7 cells, A375 cells, mouse thymus tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19649-1-AP targets Histone H1.2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, camelus bactrianus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7503 Product name: Recombinant human Histone H1.2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-213 aa of BC002649 Sequence: MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKVKKAGGTKPKKPVGAAKKPKKAAGGATPKKSAKKTPKKAKKPAAATVTKKVAKSPKKAKVAKPKKAAKSAAKAVKPKAAKPKVVKPKKAAPKKK Predict reactive species |
| Full Name | histone cluster 1, H1c |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 32-33 kDa |
| GenBank Accession Number | BC002649 |
| Gene Symbol | Histone H1.2 |
| Gene ID (NCBI) | 3006 |
| RRID | AB_10694432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16403 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. The calculated molecular weight of Histone H1.2 is 21 kDa, but modified Histone H1.2 is about 32 kDa. (PMID: 18258596)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
| IHC protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
| IP protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
| WB protocol for Histone H1.2 antibody 19649-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Histone H1.2 polyclonal antibody (Cat# 19649-1-AP) has 35 citations published in journals including Nature Neuroscience, Nature Communications, Molecular Cell, Cancer Research, Science Advances, and Cell Reports. Research fields span chromatin and histone biology, DNA damage response, cancer, inflammation, oxidative stress, viral infections, neurodegeneration, and food science/nutrition.
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol Mapping the holonomic signaling network that drives pathological changes in endotoxic shock. | ||
Cancer Res A20/TNFAIP3 Regulates the DNA Damage Response and Mediates Tumor Cell Resistance to DNA-Damaging Therapy. | ||
Nat Commun The nucleic acid binding protein SFPQ represses EBV lytic reactivation by promoting histone H1 expression | ||
