ADAM17-Specific Polyklonaler Antikörper

ADAM17-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20259-1-AP

Synonyme

ADAM17, ADAM17-Specific

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 20259-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20259-1-AP (ADAM17-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20259-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20259-1-AP (ADAM17-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 20259-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20259-1-AP (ADAM17-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 20259-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20259-1-AP (ADAM17-Specific Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20259-1-AP bindet in WB, IHC, IF, ELISA ADAM17-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ADAM metallopeptidase domain 17
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: NM_003183
• Gene symbol: ADAM17
• Gene ID (NCBI): 6868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092). The excess mass at 29 kDa predicted for unglycosylated catalytic domain(PMID: 17169570). This antibody is specific to ADAM17.

Publikationen

Species	Application	Title
human	WB,IHC	Int J Mol Med; ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.; Authors - Wei Li
human	IF	Cells; Dysregulated Cell–Cell Communication Characterizes Pulmonary Fibrosis; Authors - Jonathan S. Kurche