AHR Polyklonaler Antikörper

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• KD/KO Validated

AHR Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(50)

Rezensionen (3)

Kat-Nr. : 17840-1-AP

Synonyme

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

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  WB analysis using 17840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17840-1-AP

  WB result of AHR antibody (17840-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHR transfected HepG2 cells.

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  IP experiment of PC-3 using 17840-1-AP

  IP result of anti-AHR (IP:17840-1-AP, 4ug; Detection:17840-1-AP 1:500) with PC-3 cells lysate 1880ug.

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  IHC staining of human lung cancer using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17840-1-AP (AHR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17840-1-AP (AHR antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse small intestine using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hirngewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 44 publications below
• IHC: See 11 publications below
• IF: See 9 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

17840-1-AP bindet in WB, IHC, IF, IP, CoIP, ChIP, ELISA AHR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AHR fusion protein Ag12193
• Vollständiger Name: aryl hydrocarbon receptor
• Berechnetes Molekulargewicht: 848 aa, 96 kDa
• Beobachtetes Molekulargewicht: 105-110 kDa
• GenBank-Zugangsnummer: BC070080
• Gene symbol: AHR
• Gene ID (NCBI): 196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Publikationen

Species	Application	Title
mouse	IF	J Clin Invest; Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy; Authors - Frank L. van de Veerdonk
human	WB	J Immunother Cancer; GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer.; Authors - Jin-Li Wei ; KD Validated
human,mouse	WB	Theranostics; Activating AhR alleviates cognitive deficits of Alzheimer's disease model mice by upregulating endogenous Aβ catabolic enzyme Neprilysin.; Authors - Cheng Qian ; KD Validated
mouse	WB	Br J Pharmacol; AhR-STAT3-Hmox1/Ptgs2 signaling pathway may restrict ferroptosis and improve hMSC accumulation and efficacy in mice liver; Authors - Li Han
mouse	WB	mSystems; Impaired Intestinal Akkermansia muciniphila and Aryl Hydrocarbon Receptor Ligands Contribute to Nonalcoholic Fatty Liver Disease in Mice.; Authors - Zunji Shi
mouse	WB,IHC,IF	J Invest Dermatol; Aryl Hydrocarbon Receptor in Cutaneous Vascular Endothelial Cells Restricts Psoriasis Development by Negatively Regulating Neutrophil Recruitment.; Authors - Zhenlai Zhu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (10-03-2024)

Used this antibody for colon carcinoma cell line and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Hala (Verified Customer) (04-13-2021)

works good!

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2, U937