AKT Polyklonaler Antikörper

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• KD/KO Validated

AKT Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Kat-Nr. : 51077-1-AP

Synonyme

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 51077-1-AP

  WB result of AKT1 antibody (51077-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells.

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  WB analysis using 51077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51077-1-AP (AKT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 51077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51077-1-AP (AKT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 51077-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51077-1-AP (AKT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 51077-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51077-1-AP (AKT1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 51077-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51077-1-AP (AKT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 51077-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51077-1-AP (AKT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 51077-1-AP

  Immunofluorescent analysis of HeLa cells using 51077-1-AP (AKT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, COLO 320-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 23 publications below
• IHC: See 1 publications below

Produktinformation

51077-1-AP bindet in WB, IHC, IF, ELISA AKT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 1
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56-62 kDa
• GenBank-Zugangsnummer: BC084538
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Publikationen

Species	Application	Title
human	WB	Elife; Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.; Authors - Yixiao Zhang
human	WB	Front Oncol; Low Expression Levels of SLC22A12 Indicates a Poor Prognosis and Progresses Clear Cell Renal Cell Carcinoma.; Authors - Jiaju Xu
human	WB	Biomed Pharmacother; α-Terthienyl induces prostate cancer cell death through inhibiting androgen receptor expression.; Authors - Xia Gan
mouse	WB	Sci Rep; Aronia melanocarpa polysaccharide ameliorates inflammation and aging in mice by modulating the AMPK/SIRT1/NF-κB signaling pathway and gut microbiota; Authors - Yingchun Zhao
rat	WB	Front Cell Neurosci; Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood-Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model.; Authors - Lin-Yan Huang
human	WB	J Cell Biochem; Combined detection of estrogen and tumor markers is an important reference factor in the diagnosis and prognosis of lung cancer.; Authors - Yuquan Bai