ATG5 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ATG5 Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

3G6E6

Kat-Nr. : 60061-1-Ig

Synonyme

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 60061-1-Ig

  WB result of ATG5 antibody (60061-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HeLa cells.

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  WB analysis of K-562 using 60061-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60061-1-Ig (ATG5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60061-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60061-1-Ig (ATG5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 60061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60061-1-Ig (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60061-1-Ig (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60061-1-Ig (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60061-1-Ig (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 60061-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60061-1-Ig(ATG5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

60061-1-Ig bindet in WB, IHC, IF, ELISA ATG5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATG5 fusion protein Ag0214
• Vollständiger Name: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC002699
• Gene symbol: ATG5
• Gene ID (NCBI): 9474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection.

Publikationen

Species	Application	Title
mouse	WB	Autophagy; Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice.; Authors - Qian Huang
mouse	IF	Autophagy; The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.; Authors - Andreas Struchtrup
human,mouse	WB,IHC	Cancer Cell Int; PCK1 activates oncogenic autophagy via down-regulation Serine phosphorylation of UBAP2L and antagonizes colorectal cancer growth; Authors - Xiangyan Zhang
human	WB	Cell Biosci; A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.; Authors - Juan Yin
human	WB	Mol Med Rep; Autophagy is essential for flavopiridol‑induced cytotoxicity against MCF‑7 breast cancer cells.; Authors - Shuo Wang ; KD Validated
mouse	WB	Cells Dev; Enhanced autophagy suppresses inflammation-mediated bone loss through ROCK1 signaling in bone marrow mesenchymal stem cells.; Authors - Jingjing Zheng

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