Bcl2 Monoklonaler Antikörper
Bcl2 Monoklonal Antikörper für WB, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Maus und mehr (2)
Anwendung
WB, IF/ICC, IP, ELISA
Konjugation
Unkonjugiert
CloneNo.
1B5B9
Kat-Nr. : 66799-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | NIH/3T3-Zellen, 4T1-Zellen, C2C12-Zellen, Maushirngewebe, RAW 264.7-Zellen |
Erfolgreiche Detektion in IF/ICC | NIH/3T3-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 29 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
Produktinformation
66799-1-Ig bindet in WB, IF/ICC, IP, ELISA Bcl2 und zeigt Reaktivität mit Maus
Getestete Reaktivität | Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Bcl2 fusion protein Ag24261 |
Vollständiger Name | B-cell leukemia/lymphoma 2 |
Berechnetes Molekulargewicht | 26 kDa |
Beobachtetes Molekulargewicht | 26 kDa |
GenBank-Zugangsnummer | NM-009741 |
Gene symbol | Bcl2 |
Gene ID (NCBI) | 12043 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Bcl2 antibody 66799-1-Ig | Protokoll herunterladen |
IF protocol for Bcl2 antibody 66799-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Food Chem Crosstalk between mitochondrial dysfunction and endoplasmic reticulum stress in apoptosis: Implications for grass carp flesh quality during cold storage | ||
Phytomedicine Puerarin prevents sepsis-associated encephalopathy by regulating the AKT1 pathway in microglia | ||
Biomed Pharmacother Lychee seed polyphenol ameliorates DR via inhibiting inflammasome/apoptosis and angiogenesis in hRECs and db/db mice | ||
Redox Biol Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload. | ||
Antioxidants (Basel) Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress |