Tested Applications
| Positive WB detected in | mouse brain tissue, mouse skeletal muscle tissue, mouse heart tissue, human skeletal muscle tissue, rat brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20667-1-AP targets CaMKII delta-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | calcium/calmodulin-dependent protein kinase II delta |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | NM_001221 |
| Gene Symbol | CAMK2D |
| Gene ID (NCBI) | 817 |
| RRID | AB_10695498 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13557 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CaMKII delta, also named as CAMKD and CaMKII delta, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It can recognize most isoforms the gene encodes, from 50-72kd. It can recognize the isoform 2,3,4,9,11 of CAMK2D.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CaMKII delta-Specific antibody 20667-1-AP | Download protocol |
| IP protocol for CaMKII delta-Specific antibody 20667-1-AP | Download protocol |
| WB protocol for CaMKII delta-Specific antibody 20667-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
20667-1-AP is a CAMK2D (CaMKIIδ) affinity purification antibody with 19 total citations. Citations appear in journals including Advanced Science, Cell Death & Differentiation, Cell Death & Disease, Cell Molecular Life Sciences, Cell Signaling, International Immunopharmacology, and Hypertension Research. Research fields span cardiovascular disease (heart failure, atrial fibrillation, myocardial injury), oncology (ovarian cancer, glioma), neurology (autism, neuropathic pain, blood-brain barrier), osteoarthritis, and pulmonary calcification.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Tau Aggregation-Dependent Lipid Peroxide Accumulation Driven by the hsa_circ_0001546/14-3-3/CAMK2D/Tau Complex Inhibits Epithelial Ovarian Cancer Peritoneal Metastasis | ||
Cell Death Differ CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma | ||
Cell Death Dis miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2. | ||
Transl Psychiatry Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity. | ||
Antioxid Redox Signal CARD11-BCL10-MALT1 complex-dependent MALT1 activation facilitates myocardial oxidative stress in doxorubicin-treated mice via enhancing k48-linked ubiquitination of Nrf2 | ||
Mol Ther Nucleic Acids circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 out of 5. The reviewer reports that the antibody works well for both Western Blot (at 1:1000 dilution) and Immunofluorescence (at 1:200 dilution), confirming reliable performance across these two applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jonathan (Verified Customer) (11-11-2019) | Works well for WB (1:1000) and IF (1:200)
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