CD11b Monoklonaler Antikörper

CD11b Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(2)

CloneNo.

M1/70

Kat-Nr. : CL488-65055

Synonyme

CR3, CR3A, integrin alpha M, Itgam, Ly 40

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  IF Staining of BV-2 using CL488-65055

  Immunofluorescent analysis of (4% PFA) fixed BV-2 cells using CoraLite® Plus 488 CD11b antibody (CL488-65055, Clone: M1/70 ) at dilution of 1:400.

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  IF Staining of mouse splenocytes using CL488-65055

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD11b (CL488-65055, Clone: M1/70) at dilution of 1:400.

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  FC experiment of mouse bone marrow cells using CL488-65055

  1x10^6 mouse bone marrow cells were surface stained with APC Anti-Mouse Ly-6G/Ly-6C (Gr-1), and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (M1/70) (CL488-65055, Clone: M1/70) or CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2). Cells were not fixed.

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  FC experiment of mouse bone marrow cells using CL488-65055

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (M1/70) (CL488-65055, Clone:M1/70) (red) or CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Maus-Splenozyten, BV-2 cells
• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

CL488-65055 bindet in IF/ICC, FC CD11b und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C57BL/10 Mausmilz-T-Zellen und Concanavalin A-aktivierte C57BL/10-Splenozyten
• Vollständiger Name: integrin alpha M
• Berechnetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: BC156991
• Gene symbol: CD11b
• Gene ID (NCBI): 16409
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publikationen

Species	Application	Title
mouse	FC	Vet Res; Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis; Authors - Luqiu Feng
	IF	Biomed Pharmacother; Mitochondrion-NLRP3 inflammasome activation in macrophages: A novel mechanism of the anti-inflammatory effect of Notopterygium in rheumatoid arthritis treatment; Authors - Xiangxiang Liu