CD11b Monoklonaler Antikörper
CD11b Monoklonal Antikörper für FC
Wirt / Isotyp
Maus / IgG1, kappa
Getestete Reaktivität
human
Anwendung
FC
Konjugation
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
ICRF44
Kat-Nr. : CL750-65116
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in FC | humane PBMCs |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL750-65116 bindet in FC CD11b und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| Wirt / Isotyp | Maus / IgG1, kappa |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Rheumatoide Synovialzellen und humane Monozyten |
| Vollständiger Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Berechnetes Molekulargewicht | 1152 aa, 127 kDa |
| GenBank-Zugangsnummer | BC096346 |
| Gene symbol | CD11b |
| Gene ID (NCBI) | 3684 |
| Konjugation | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Reinigungsmethode | Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.09% sodium azide and 0.5% BSA |
| Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Hintergrundinformationen
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| FC protocol for CL Plus 750 CD11b antibody CL750-65116 | Download protocol |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Cyril (Verified Customer) (11-19-2024) | It is complicated to conclude on the use of this antibody. Indeed, the laser of our Attune NxT does not allow to detect the Coralyte 750 (maximum 17% emission). We will soon test another format for the project.
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