CD11b Monoklonaler Antikörper

CD11b Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

human, Maus

Anwendung

FC

Konjugation

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

M1/70

Kat-Nr. : CPY5-65055

Synonyme

CR3, CR3A, integrin alpha M, Itgam, Ly 40

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  FC experiment of mouse bone marrow cells using CPY5-65055

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug PerCP-Cyanine5.5 Anti-Mouse CD11b (M1/70) (CPY5-65055, Clone:M1/70) or unstained. Cells were then stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (CL647-65140, Clone: RB6-8C5). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CPY5-65055 bindet in FC CD11b und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C57BL/10 Mausmilz-T-Zellen und Concanavalin A-aktivierte C57BL/10-Splenozyten
• Vollständiger Name: integrin alpha M
• Berechnetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: BC156991
• Gene symbol: CD11b
• Gene ID (NCBI): 16409
• Konjugation: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 482 nm / 690 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).