CD11b Monoklonaler Antikörper

CD11b Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(5)

CloneNo.

ICRF44

Kat-Nr. : FITC-65116

Synonyme

ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha

---

• 

  FC experiment of human PBMCs using FITC-65116

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11b (FITC-65116, Clone:ICRF44). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65116

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11b (FITC-65116, Clone:ICRF44). Cells were not fixed. Monocytes were gated.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 5 publications below

Produktinformation

FITC-65116 bindet in FC CD11b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rheumatoide Synovialzellen und humane Monozyten
• Vollständiger Name: integrin, alpha M (complement component 3 receptor 3 subunit)
• Berechnetes Molekulargewicht: 1152 aa, 127 kDa
• GenBank-Zugangsnummer: BC096346
• Gene symbol: CD11b
• Gene ID (NCBI): 3684
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publikationen

Species	Application	Title
human	FC	Oncogene; Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells; Authors - Ying Zhan
human	FC	Cells; Exosomes from Human Omental Adipose-Derived Mesenchymal Stem Cells Secreted into Ascites Promote Peritoneal Metastasis of Epithelial Ovarian Cancer; Authors - Qingxi Qu
	FC	Int Immunopharmacol; Neutrophil PPIF exacerbates lung ischemia-reperfusion injury after lung transplantation by promoting calcium overload-induced neutrophil extracellular traps formation; Authors - Wensi Wu
human	FC	Tissue Cell; Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization; Authors - Lei Zhu
human	FC	Adv Sci (Weinh); Enhancing Tendon Regeneration: Investigating the Impact of Topography on the Secretome of Adipose-Derived Stem Cells; Authors - Qiuzi Long