CD11c Monoklonaler Antikörper

CD11c Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Armenian Hamster / IgG

Getestete Reaktivität

Maus

Anwendung

IF/ICC, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

N418

Kat-Nr. : CL488-65130

Synonyme

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

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  IF Staining of mouse splenocytes using CL488-65130

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD11c antibody (CL488-65130, Clone: N418 ) at dilution of 1:400.

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  FC experiment of mouse splenocytes using CL488-65130

  1X10^6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11c (CL488-65130, Clone: N418) or 0.25 ug isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-65130 bindet in IF/ICC, FC CD11c und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Armenian Hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Dendritische Mausmilzzellen
• Vollständiger Name: integrin alpha X
• GenBank-Zugangsnummer: BC167225
• Gene symbol: CD11c
• Gene ID (NCBI): 16411
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Publikationen

Species	Application	Title
mouse	IF	Acta Pharm Sin B; Free fatty acid receptor-4 regulates T-cell-mediated allogeneic reaction through activating an aryl hydrocarbon receptor pathway; Authors - Maxwell Duah