CD16/32 Monoklonaler Antikörper

CD16/32 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2a, lambda

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

93

Kat-Nr. : CL647-65057

Synonyme

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

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Galerie der Validierungsdaten

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  IF Staining of mouse splenocytes using CL647-65057

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647 CD16/32 antibody (CL647-65057, Clone: 93 ) at dilution of 1:100.

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  FC experiment of mouse splenocytes using CL647-65057

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3ε (FITC-65060, Clone: 145-2C11) and 5 ul CoraLite® Plus 647 Anti-Mouse CD16/32 (CL647-65057, Clone: 93). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-65057

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3ε (FITC-65060, Clone: 145-2C11) and CoraLite® Plus 647 conjugated Rat IgG2a isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65057 bindet in IF, FC CD16/32 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VH81X Tg-B-Zellen und T220-Stromazelllinie
• Vollständiger Name: Fc receptor, IgG, low affinity IIb
• GenBank-Zugangsnummer: BC038070
• Gene symbol: Fcgr2b
• Gene ID (NCBI): 14130
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 665 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.