Tested Applications
| Positive FC detected in | human PBMCs, cynomolgus PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-FcA65575 targets CD20 in FC applications and shows reactivity with human, cynomolgus monkey samples.
| Tested Reactivity | human, cynomolgus monkey |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Human tonsillar B cells Predict reactive species |
| Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
| Calculated Molecular Weight | 297 aa, 33 kDa |
| GenBank Accession Number | BC002807 |
| Gene Symbol | CD20 |
| Gene ID (NCBI) | 931 |
| RRID | AB_3749635 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11836-1 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD20 antibody PE-FcA65575 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PE-FcA65575 is the FcZero-rAb® Anti-Human CD20 (2H7) Rabbit Recombinant Antibody. It has a total of 1 citation, published in Advanced Science (2025). The cited study investigated spatial transcriptional dynamics of CD74⁺ B cells in tertiary lymphoid structures and their role in immune evolution within penile squamous cell carcinoma, placing the research in the fields of immunology and oncology. The antibody was applied in flow cytometry for human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Spatial Transcriptional Dynamics of CD74⁺ B Cells in Tertiary Lymphoid Structures Drive Immune Evolution in Penile Squamous Cell Carcinoma. |
