PD-L1/CD274 Monoklonaler Antikörper

PD-L1/CD274 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus und mehr (1)

Anwendung

IHC, IF, FC

Konjugation

APC Fluorescent Dye

Publikationen(4)

CloneNo.

10F.9G2

Kat-Nr. : APC-65073

Synonyme

Cd274, PD-L1, 10F.9G2, B7 H1, PD L1

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  FC experiment of mouse splenocytes using APC-65073

  1X10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) BALB/C mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CD274 (PD-L1, B7-H1) (10F.9G2) (APC-65073, Clone:10F.9G2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 2 publications below

Produktinformation

APC-65073 bindet in IHC, IF, FC PD-L1/CD274 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD274 antigen
• GenBank-Zugangsnummer: BC066841
• Gene symbol: PD-L1
• Gene ID (NCBI): 60533
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publikationen

Species	Application	Title
	FC	Cell Signal; Cancer-associated fibroblasts-induced remodeling of tumor immune microenvironment via Jagged1 in glioma; Authors - Qing Zhang
mouse	FC	Adv Mater; Elucidation of Spatial Cooperativity in Chemo-Immunotherapy by a Sequential Dual-pH-Responsive Drug Delivery System; Authors - Shihao Wang
human	IHC,IF	Cell Metab; Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1; Authors - Yizeng Fan
		Adv Mater; An OMV-Based Nanovaccine as Antigen Presentation Signal Enhancer for Cancer Immunotherapy; Authors - Yichao Lu